We use RNA-seq to characterize the animal and plant transcriptome and construct complete gene annotations including coding genes, non-coding genes, alternative splicing, gene fusion, novel gene function. We also discover the role of non-coding RNAs in regulation of gene expression.
We integrate multi-omics data including genome, transcriptome, methylatome and tranlatome data to construct bioinformatics database, and develop pipeline and software for processing sequencing data.
We use Ribo-seq to understand protein translation status, translation rate and expression abundance at the genome level. We also identify translated ORFs from non-coding RNAs, and explore the biological functions of their encoded microproteins.