| Gene ID | Gene Name | Biotype | Dbxref | Location |
|---|
| # | Primer | Temperature | GC Content | End stability | Product Size | Location |
|---|
| General settings | |
|---|---|
| PRIMER_PRODUCT_SIZE_RANGE | |
| PRIMER_NUM_RETURN | |
| Primer size | |
| PRIMER_MIN_SIZE | |
| PRIMER_OPT_SIZE | |
| PRIMER_MAX_SIZE | |
| Primer GC content | |
| PRIMER_MIN_GC | |
| PRIMER_MAX_GC | |
| PRIMER_GC_CLAMP | |
| Primer melting temperature | |
| PRIMER_MIN_TM | |
| PRIMER_OPT_TM | |
| PRIMER_MAX_TM | |
| Self-binding (primer-dimer and hairpins) | |
| PRIMER_MAX_SELF_ANY_TH | |
| PRIMER_MAX_SELF_END_TH | |
| PRIMER_PAIR_MAX_COMPL_ANY_TH | |
| PRIMER_PAIR_MAX_COMPL_END_TH | |
| PRIMER_MAX_HAIRPIN_TH | |
| PolyX and others | |
| PRIMER_MAX_END_STABILITY | |
| PRIMER_MAX_POLY_X | |
| PRIMER_MAX_NS_ACCEPTED | |
| PRIMER_PAIR_MAX_DIFF_TM | |